Multiple myeloma gene-modified tumor vaccine-induced anti-tumor response in vivo experimental study.doc

 PAGE \* MERGEFORMAT 15 Multiple myeloma gene-modified tumor vaccine-induced anti-tumor response in vivo experimental study Author: Su-Ping Ren, Li-Sheng Wang, Guo Qiang, Wang, Jia Xiang-Xu, Juan Xu, Heng-Xiang Wang, Chu-Tse Wu Abstract The purpose of this study was to evaluate NOD / SCID mice with subcutaneous xenograft model of multiple myeloma in vivo gene-modified tumor vaccine induced anti-tumor response results. First of all to the NOD / SCID mice by intraperitoneal injection of human peripheral blood lymphocytes in the body in order to rebuild the human immune system, and then inoculated subcutaneously with γ-ray inactivated gene-modified myeloma cell line sko-007 (expressing green fluorescent protein, or p53, GM-CSF and B7-1 gene), to PBS as a control, the last living sko-007 cells implanted in attack. The results showed that, compared with the control group vaccinated infected with adenovirus Ad-p53/GM-CSF/B7-1 the sko-007 cells can inhibit tumor growth, pathologic analysis showed that tumor fibrous tissue hyperplasia with an increase in diffuse necrosis, vascular hyperplasia remarkable. Immunohistochemical staining showed some tumor foci in T lymphocytes. Conclusions: p53, GM-CSF and B7-1 gene-modified myeloma cells can induce anti-tumor immune response, it is possible for human multiple myeloma immunotherapy. Keywords: Multiple myeloma Multiple myeloma (MM) remains an incurable malignancy despite advances in chemotherapy. Myeloablative chemotherapy followed by allogeneic or autologous hematopoietic stem cell transplantation has increased the incidence of complete remission, but almost all patients achieving complete remission ultimately experience relapse [1-3]. Because these chemotherapies have only limited value, alternative strategies are needed to solve these problems. Immunotherapy may represent a means of maintaining complete remission. Based on the fact that myeloma cells contain a multitude of tumor ant