Musca domestica molecular structure and antimicrobial spectrum of antimicrobial peptide research.docVIP

 PAGE \* MERGEFORMAT 10 Musca domestica molecular structure and antimicrobial spectrum of antimicrobial peptide research Author: Zhou Wen SHEN Ping Zhi-Guang Tu Yin Chen Hui Meng Jiangping Wang a soldier in the Ru-Shan Chen Liang Lu Chen Zhenggeng [Abstract] Objective To study the molecular structure of Musca domestica antimicrobial peptides and antimicrobial spectrum, to find effective natural broad-spectrum antimicrobial peptide. Methods Musca domestica has been made of damage infected tissue induced by immunization with peptide separation and purification of protein purification techniques, determination of antibacterial activity and antibacterial spectrum, nano-electrospray ionization time of flight mass spectrometry sequence of amino acids. Results housefly tissue extract by protein purification, to be a purified antimicrobial active ingredients, nano-electrospray ionization time of flight mass spectrometry sequencing, are two amino acid sequences of peptides: peptides 1.QPNLYYNK and peptide 2. PNNVYYTK. Through the NCBInr protein database search found no matches with the protein sequence. The antimicrobial peptide isolated from clinical samples of 11 kinds of strains of Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, Proteus vulgaris, shape Citrobacter, Klebsiella pneumoniae, Enterobacter cloacae, Shigella flexneri, Table Portugal bacteria, methicillin-resistant Staphylococcus aureus (MRSA) and Candida albicans the MIC were: 3.89,2.22,4.44,6.67,3.33,6.67,5.0,6.67,4.44,4.44 and 13.33μ mol / L. Conclusions obtained in this peptide is a new antimicrobial peptide, there are broad-spectrum antibacterial activity against Gram-negative bacteria and gram-positive bacteria have a certain extent. [Keywords:] housefly antimicrobial peptide; antibacterial spectrum; molecular structure; amino acid sequencing ABSTRACT Objective To investigate the broad spectrum and high effective natural Musca domestica antibacterial peptide, and study its molecular str