Mushroom C91-3 cDNA library construction and identification of.doc

 PAGE \* MERGEFORMAT 10 Mushroom C91-3 cDNA library construction and identification of Of: Wang Xiaoli, Zhong Min Tao, GUIZHOU EDUCATIONAL Cao Jing, Ning Hong, Huang Min [Abstract] Objective To construct the mushroom C91-3 cDNA library. Method used to collect low-speed centrifugation C91-3 mushroom mycelium mycelium in the fermentation broth, extraction of total RNA, amplified by nested PCR 5SrRNA 2 fragments of non-transcribed spacer region and DNA sequencing . purified mRNA, the mRNA as a template for reverse transcription reaction product and pBlueScript II SK (+ vector, and transformed E.coil DH10B, testing capacity and inserts. the results of the initial mushroom C91-3cDNA library storage capacity of 7.2 106cfu, insert length of 0.4 ~ 3.4kb, an average of 1.5kb. Conclusion The construction of mushrooms and C91-3 cDNA library was used to study the mushrooms for protein coding genes. [Keywords:] mushrooms, cDNA library, 5SrRNA non-transcribed spacer 2 from the tree, storage As a commonly used medicinal mushroom fungus in the prevention, treatment, and have an important value in malignant tumors, and its polysaccharide extract mainly -1, 3 bond connecting the main chain and -1, 6 branched glucan bond connectivity , can improve immune function and indirectly inhibit tumor growth [1,2], the outstanding anti-cancer, enhance immunity and ease the effects of chemotherapy reactions [3,4], is now officially be used as drugs used in chemotherapy clinical, mushrooms have been as anti-cancer plant resources [5]. The growth cycle of mushrooms 2 to 3 months, and biological fermentation culture can be harvested 7 ~ 10 d significantly reduced to a test cycle, and the fermentation broth and amino acid composition of the polysaccharides in the mushroom fruiting bodies and the analysis of reported results. Therefore, over the years our group has been mushroom broth protein for the study. preliminary study confirmed that mushrooms C91-3 fermentation broth has a