Myanmar viper venom plasminogen Faa Isolation and biological activity.doc

 PAGE \* MERGEFORMAT 20 Myanmar viper venom plasminogen Faa Isolation and biological activity Of: Chen Huizhen, Liangxiu Xia, QIU Peng-xin, Chen tree [Abstract] [aim] viper venom from Myanmar in the purified plasminogen F aa and to study their fibrinolytic activity. [Method] Myanmar viper venom plasminogen F aa through the ion-exchange chromatography and gel chromatographic separation procedures are , SDSelectrophoresis obtaining the molecular weight measured by obtaining the fibrin plate method to measure fibrinolytic activity, SDSelectrophoresis test obtaining the hydrolysis of fibrinogen and fibrin specificity. by subcutaneous injection in mice measured F aa F aa The local hemorrhagic activity. take 42 Kunming mice, body weight (20 + - 2) g, were randomly divided into 7 groups, n = 6, 0.1 mL subcutaneous injection of F aa (5 dose group: concentration of saline were made into 0.025,0.05,0.075,0.1,0.125 mg / mL), saline, and crude venom (0.1 mg / mL) as control mice were sacrificed after .6 h, bleeding from the subcutaneous side of the measuring point size, calculate the minimum hemorrhagic dose (MHD). [results] obtained through the three steps separating the venom plasminogen F aa, its molecular weight is 69 000 u.F aa fibrin plate and heated in the fibrin plate have shown that fibrinolysis activity. fibrinogen and F aa (0.2 g / L) insulation after, A @ and B chains, respectively, in 5 min and 6 h after the complete hydrolysis. -chain in the 24 h after partial hydrolysis. F aa hydrolysis of fibrin process and fiber similar to the hydrolysis process of fibrinogen. Snake Venom Plasminogen F aa (0.025,0.05,0.075,0.1,0.125 mg / mL) of the skin bleeding spot diameter (mm) were 7.7 + - 1.0,9.9 + - 1.4,10.5 + - 1.8 , 12.7 + - 1.4,13.5 + - 1.3. minimal bleeding dose (MHD) was 6.0 g. [Conclusion] F aa is a direct degradation of fibrin and has hemorrhagic activity of the @ - fiber protein. [Keywords:] plasmin; venom; Viper Abstract: [Objective] T