网站大量收购独家精品文档,联系QQ:2885784924

Myanmar viper venom plasminogen Faa Isolation and biological activity.doc

Myanmar viper venom plasminogen Faa Isolation and biological activity.doc

  1. 1、本文档共20页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
Myanmar viper venom plasminogen Faa Isolation and biological activity

 PAGE \* MERGEFORMAT 20 Myanmar viper venom plasminogen Faa Isolation and biological activity Of: Chen Huizhen, Liangxiu Xia, QIU Peng-xin, Chen tree [Abstract] [aim] viper venom from Myanmar in the purified plasminogen F aa and to study their fibrinolytic activity. [Method] Myanmar viper venom plasminogen F aa through the ion-exchange chromatography and gel chromatographic separation procedures are , SDSelectrophoresis obtaining the molecular weight measured by obtaining the fibrin plate method to measure fibrinolytic activity, SDSelectrophoresis test obtaining the hydrolysis of fibrinogen and fibrin specificity. by subcutaneous injection in mice measured F aa F aa The local hemorrhagic activity. take 42 Kunming mice, body weight (20 + - 2) g, were randomly divided into 7 groups, n = 6, 0.1 mL subcutaneous injection of F aa (5 dose group: concentration of saline were made into 0.025,0.05,0.075,0.1,0.125 mg / mL), saline, and crude venom (0.1 mg / mL) as control mice were sacrificed after .6 h, bleeding from the subcutaneous side of the measuring point size, calculate the minimum hemorrhagic dose (MHD). [results] obtained through the three steps separating the venom plasminogen F aa, its molecular weight is 69 000 u.F aa fibrin plate and heated in the fibrin plate have shown that fibrinolysis activity. fibrinogen and F aa (0.2 g / L) insulation after, A @ and B chains, respectively, in 5 min and 6 h after the complete hydrolysis. -chain in the 24 h after partial hydrolysis. F aa hydrolysis of fibrin process and fiber similar to the hydrolysis process of fibrinogen. Snake Venom Plasminogen F aa (0.025,0.05,0.075,0.1,0.125 mg / mL) of the skin bleeding spot diameter (mm) were 7.7 + - 1.0,9.9 + - 1.4,10.5 + - 1.8 , 12.7 + - 1.4,13.5 + - 1.3. minimal bleeding dose (MHD) was 6.0 g. [Conclusion] F aa is a direct degradation of fibrin and has hemorrhagic activity of the @ - fiber protein. [Keywords:] plasmin; venom; Viper Abstract: [Objective] T

您可能关注的文档

文档评论(0)

jiupshaieuk12 + 关注
实名认证
内容提供者

该用户很懒,什么也没介绍

版权声明书
用户编号:6212135231000003

1亿VIP精品文档

相关文档