Mycobacterium tuberculosis Mechanisms of drug resistance.doc

 PAGE \* MERGEFORMAT 18 Mycobacterium tuberculosis Mechanisms of drug resistance [Keywords:] Mycobacterium tuberculosis; gene; mutation; drug resistance TB is a serious harm to people’s health and infectious diseases. Resistant TB, especially multi-drug resistant tuberculosis (MDR-TB) led to the emergence and spread of TB is an important reason for increased incidence. Mycobacterium tuberculosis can not be mediated by plasmid guidance received from the other bacterial drug resistance, so resistance is chromosome-mediated molecular basis of TB drug resistance. At present the mechanism of drug resistance of Mycobacterium tuberculosis focused on drug targets and related gene mutation on. now Mycobacterium tuberculosis on the major anti-TB drug resistance mechanisms reviewed the research progress. 1 isoniazid (INH) Research has shown that INH-resistant Mycobacterium tuberculosis involves multiple genetic changes: katG, inhA, kasA, ndh and the oxyR-ahpC gene was cloned area. 1. 1 katG catalase gene is a peroxidase (catalase-peoxidase) encoding genes. Isoniazid was within the Mycobacterium tuberculosis catalase - peroxidase activity and activation products act on the enoyl-ACP reductase (of the fatty acid synthase II system, which can promote short-chain fatty acid synthesis fatty acid), inhibit acid bacteria and cell wall biosynthesis. katG gene separated by 44 bp upstream and furA gene (a kind of iron regulatory protein encoded Mycobacterium tuberculosis gene, may be katG gene promoter and regulation of its expression) connected to the downstream separated by 2794 bp embC gene linked to length of 2223 nucleotides. cause INH resistance is mainly due to point mutations in katG gene, deletion, insertion, complete absence of INH resistant strains accounted for only 7% ~ 24%. katG gene mutations are common AGC → ACC 315 and 463 315 CGG → CTG.katG gene mutations usually result in a hydrogen peroxide oxide activity decreased, resulting in high levels of res