Müller cells in rat retinal capillary endothelial cell proliferation and migration of.docVIP

 PAGE \* MERGEFORMAT 34 Müller cells in rat retinal capillary endothelial cell proliferation and migration of Authors: Guo Bin, Wang Ying Li, Yan-Nian Hui, Wang Yu-sheng, Ji-Xian 【Abstract】 Objective: The MACS primary cultured rat retinal microvascular endothelial cells (RMEC), observation of co-cultured Müller cells to retinal capillary endothelial cell proliferation and migration effects. Methods: Immunomagnetic cell sorting separation of rat retinal capillary endothelial cells cultured in the conditioned medium of growth factor Ⅷ using antibody staining cells were identified using flow cytometry and trypan blue dye Analysis of passage in the microvascular endothelial cell purity and cell activity. Traditional methods and identify Müller cells in culture. Using different pore size microporous membrane culture chamber to carry out rat retinal capillary endothelial cells and Müller cell separation were cultured in the control group in non-Müller cells in culture environments. Curve of cells painted to reflect cell proliferation, and used flow cytometry cell cycle. Calculate the phase contrast microscope attached to migrate through the porous membrane at the back of the endothelial cells. Results: bead separation method to obtain a purity of 97.13% of rat retinal microvascular endothelial cells, cell viability up to 92%, the first factor Ⅷ antibody staining positive. And Müller cells were cultured in normal culture RMEC may be as early as 2 ~ 3d into the growth plateau. RMEC in the S phase and G2-phase fraction increased, G1 phase was reduced. S, G2, and G1 phase percentage, in the co-culture group of 24h were 44.0%, 11.2% and 44.8%, 48h, respectively 42.3%, 10.9% and 46.8%; the control group 24h culture were 41.3%, 4.9% and 53.8% , 48h, respectively 40.1%, 4.7% and 55.2%. Increase the number of migrating cells were cultured 6h migrating to the membrane under the endothelial cell count 12.2 ± 2.5 Ge, 12h to 51.7 ± 23.4 Ge in the control culture 6h m