Na K-ATP enzyme β1 subunit associated with tumor cell growth.doc

 PAGE \* MERGEFORMAT 11 Na K-ATP enzyme β1 subunit associated with tumor cell growth Author: Xiong Zhu-juan Lin Ping Zhang Jie, Wang Xiu-Jie Wang Qi Ren Jing-Jing Yang Hongliang Wang Jing Wu Yaying Abstract [Objective] To investigate the Na, K-ATP enzyme β1 subunit (ATP1B1) and tumor cell proliferation and differentiation of the relationship, and exogenous ATP1B1 on tumor cells. [Methods] RT-PCR detection of tumor cell lines, normal peripheral blood mononuclear cells in the mRNA levels of ATP1B1, as well as by lipopolysaccharide (LPS) to promote cell proliferation, dimethyl sulfoxide (DMSO)-induced tumor cell differentiation of post-ATP1B1 mRNA level changes, and to observe increased by transfection ATP1B1 expression in tumor cells after the growth of cells. [Results] The tumor cell lines in the mRNA levels of ATP1B1 was significantly higher than normal mononuclear cells (P lt;0.05), promote cell proliferation of cell in the ATP1B1 the mRNA level increased (P lt;0.05), induce differentiation and then decreases (P lt;0.05) , import exogenous ATP1B1 the tumor cells significantly inhibited (P lt;0.05). [Conclusion] Na, K-ATP enzyme β1 subunit could be reflecting the functional state of cellular proliferation and metabolism of tumor cells in particular, an important indicator of biological therapy for cancer provide an important new strategy for experimental evidence. Keywords: Na K-ATP enzyme β1 subunit-induced differentiation of tumor cell proliferation Abstract: [Purpose] To explore the relationship between Na, K-ATPase β1-Subunit (ATP1B1) and tumor cell in proliferation and differentiation, as well as the effect of ATP1B1 introduction. [Methods] The levels of ATP1B1 mRNA were detected by RT -PCR in both of tumor cell lines and normal peripheral blood monocyte, and the changing of ATP1B1 mRNA were analyzed in the proliferation-cells stimulated by LPS and the differentiation-cells induced by DMSO. Moreover, survival of cells transfect