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Nano-immunomagnetic beads combined RTnestPCR Detection of micrometastasis in peripheral blood of pancreatic cancer the sensitivity and specificity.doc

Nano-immunomagnetic beads combined RTnestPCR Detection of micrometastasis in peripheral blood of pancreatic cancer the sensitivity and specificity.doc

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Nano-immunomagnetic beads combined RTnestPCR Detection of micrometastasis in peripheral blood of pancreatic cancer the sensitivity and specificity

 PAGE \* MERGEFORMAT 12 Nano-immunomagnetic beads combined RTnestPCR Detection of micrometastasis in peripheral blood of pancreatic cancer the sensitivity and specificity Of: Hu Liang, Jia-Hua Zhou, Yu Ze ago, Tao Tao can be [Abstract] Objective: pancreatic cancer micrometastasis model of nano-immunomagnetic beads combined RT nest PCR detection sensitivity and specificity of micrometastasis. METHODS: A pancreatic cancer cells PANC 1 dilution were added to normal peripheral blood nuclear cells, and then add Fc proportion of non-specific receptor antagonist, and epithelial cells of EP CAM antibody, 4 and mix incubated under 30 min, by positive sorting separation column was extracted after RNA, RT nest PCR assay cancer gene human telomerase subunits (h TERT) and carcinoembryonic antigen (CEA). Results: The nano-immunomagnetic beads and RT nest PCR, an average of 1 107 peripheral blood mononuclear cells could be detected in a benign tumor cells .10 disease control group showed no expression. Conclusion: The combined RT nest PCR immunomagnetic beads is both high sensitivity and specificity of micrometastasis detection methods, can significantly increase the tumor detection rate of early micrometastasis. [Keywords:] pancreatic cancer; micrometastasis; immunomagnetic beads; reverse transcription-nested polymerase chain reaction The incidence of pancreatic cancer increased year by year trend, 90% of patients died within 1 year after diagnosis .5-year survival rate of only 1% to 3%. Although the primary tumor resection and postoperative radiotherapy and chemotherapy can prolong cancer patients survival rate, but the 5-year survival rate is still low, around 4%, even though patients underwent radical resection, 80% of patients relapse within 2 years [1]. study found that with the micro-metastasis [2]. Detection of micrometastasis of tumor specimens with lymph node, peripheral blood, bone marrow, portal vein blood, peritoneal fluid, peritoneal, pleural la

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