Nanocomposite hydroxyapatite (nHA) root canal filling material applied basic research.doc

 PAGE \* MERGEFORMAT 14 Nanocomposite hydroxyapatite (nHA) root canal filling material applied basic research [Abstract] Objective of the nano-hydroxyapatite composite root filling material and the traditional root canal filling material (filling with compound thymol were bulk formocresol cytotoxic compound preparation, root canal and the seal of the Comparative Study of Antimicrobial Resistance , nano-hydroxyapatite composite evaluation of cell toxicity, root canal microleakage and antibacterial effects. materials and methods of cell toxicity: in vitro cultured osteoblasts, nano hydroxyapatite composite materials extraction, and to culture medium for the negative control group, filled with compound thymol powder formocresol composite paste for the positive control group. according to ISO standard method used in vitro MTT cytotoxicity test, the test material in different extraction time and adult bone extracts cell contact with the absorbance values #8203;#8203;of 1,3,5,7 days to evaluate the cell toxicity. root canal seal of the experiment: 10 recently extracted single root teeth were randomly divided into 2 groups according to conventional methods of tooth root canal preparation. experimental group was 40% nano-hydroxyapatite composite paste filling, the control group was filled with compound thymol composite powder formocresol paste filling. take X-ray film to ensure that each group tooth roots in vitro experiments are perfect filling, fully hardened after the microleakage test. microleakage of improved glucose monitoring devices for root canal microleakage test, 30 days after the results measured by the quantitative analysis of glucose. antimicrobial activity test: nutritious dip broth medium repeatedly rubbing a cotton swab infected root canals, the bacterial inoculation, isolation, identification, training. culture results for the variant streptococcus, staphylococcus aureus can use two kinds of bacteria, blood culture medium, respectively, the general