Naoxintong THP1 in human monocyte-derived macrophage scavenger receptor expression.doc

 PAGE \* MERGEFORMAT 14 Naoxintong THP1 in human monocyte-derived macrophage scavenger receptor expression Of: Liu Qian, Wang Dongqi, Leixin Jun, Chang-Cong Cui, Li Hongbing, Liu Shengqiang [Abstract] Objective To Atorvastatin as standard control of proprietary Chinese medicines Naoxintong THP 1 human monocyte-derived macrophage scavenger receptor CD36 and SR A expression. Methods Human monocyte-derived THP 1 macrophages and 50mg / L oxLDL coculture 0,8,16,24 h, flow cytometry cell surface at each time point and SR A scavenger receptor CD36 expression. Naoxintong different concentrations (0.125,0.25,0.5 , 1g / L) and 1mol / L atorvastatin intervention THP 1 monocytes were derived macrophage medium was changed after 12h, were added to the liquid and the concentration of 50mg / L oxLDL incubated 24h, testing the expression of CD36 and SR A volume, statistical analysis and different concentrations of Naoxintong 1mol / L atorvastatin on the expression of CD36 and SR A. The results clearly promote human THP 1 oxLDL monocyte derived macrophages the expression of CD36 and SR A, Naoxintong dose-dependent inhibition of the expression of CD36 and SR A, 1g / L Naoxintong most obvious effect (decreased by 22.3%, 25.0%, P lt;0.05), 1mol / L atorvastatin significantly inhibited both expression (decreased 63.3%, 70.5%, P lt;0.05). Conclusion medicine Naoxintong THP 1 mild inhibition of monocyte-derived macrophage expression of scavenger receptor function, this effect was weaker than that of atorvastatin. [Keywords:] THP 1 macrophages Naoxintong, CD36, SRATHP 1 ABSTRACT: Objective To investigate the effect of Chinese prepared medicine Naoxintong on scavenger receptors CD36 and SR A expression of human THP 1 monocyte derived macrophages. Methods Human THP 1 monocyte derived macrophages were coincubated with 50mg / L oxLDL for 0h, 8h, 16h and 24h . Cell surface scavenger receptors CD36 and SR A expression were assessed by flow cytometry. Human THP 1 monocyte derived