Natamycin Optimization of HPLC detection wavelength.doc

 PAGE \* MERGEFORMAT 9 Natamycin Optimization of HPLC detection wavelength [Abstract] Objective To determine the HPLC detection of Natamycin the best wavelength of spectrophotometric scanning and HPLC determination of relationships. Methods content of 1 ~ 2000μ g/ml natamycin methanol solution of the full-wavelength scanning, analysis of the wavelength range of linear relationship between different concentrations to determine the best linear relationship between the HPLC detection wavelength as the wavelength and to determine the wavelength under the condition of theoretical plates, linear range, precision, stability and recovery. The results provided USP26 version of natamycin HPLC detection wavelength is 303nm, but different concentrations of Natamycin full wavelength scan showed, 800μ g/ml the following concentrations of natamycin at 303nm at R2 = 0.383,250 nm at 0.993 . Be Natamycin HPLC detection wavelength of 250nm, after adjustment, 5 ~ 2000μ g/ml within the R2 of 0.99995; 303nm Department under the same conditions R2 above 0.99 in the concentration range of only 5 ~ 200μ g/ml. Conclusion 250nm is natamycin HPLC detection of a more appropriate wavelength; full wavelength scan analysis than a simple linear relationship between the use of the maximum absorption wavelength of the HPLC detection wavelength selection has a better guidance and practical value. [Keywords:] Natamycin; high-performance liquid chromatography; wavelength Natamycin (1) anti-fungal polyene macrolide antibiotics, is an important biological preservatives [1]. Method of its determination spectrophotometer analysis, colorimetric analysis, microbial analysis, titration analysis, paper chromatography, thin layer chromatography and HPLC analysis of [2 ~ 4]. HPLC separation method which is good, can accurately quantitative, analysis time is short, is the most widely used method. USP26 version under the HPLC analytical method for the detection of the wavelength to 303nm [5]. The company w