Neonatal hypoxic ischemic encephalopathy calcium balance disorders and the correlation of brain injury.doc

 PAGE \* MERGEFORMAT 22 Neonatal hypoxic ischemic encephalopathy calcium balance disorders and the correlation of brain injury [Abstract] Objective To determine the neonatal hypoxic ischemic encephalopathy (hypoxic-ischemic encephalopathy, HIE) in children with red blood cells and red blood cell membrane calcium concentration inside and outside the Ca 2 + -Mg 2 + -ATPase activity, the Institute detection index and the relationship between brain injury and to explore whether there when the calcium treatment of hypocalcemia, to maximize the efficacy of HIE, to reduce death and disability. Methods 86 cases detected by flow cytometry students to varying degrees children with hypoxic ischemic encephalopathy in patients with red blood cell Ca 2 + concentration to I-STAT1-Analyzer determination of plasma free calcium, measured by chemical assay of red cell membrane Ca 2 + -Mg 2 + -ATPase activity ; to 30 cases of newborns as a control group, both inhalation and ingestion of amniotic fluid syndrome. HIE group, some children will be divided into groups with and without calcium supplement calcium supplement group, the end of treatment when the added calcium detected again after groups of related indicators. Results HIE acute phase plasma free calcium concentration were significantly lower than the control group, (P lt;0.05), red blood cell Ca 2 + concentration were significantly higher (P lt;0.05) erythrocyte membrane Ca 2 + -Mg 2 + -ATPase activity was significantly lower than the control group (P lt;0.05); these indicators change and severity of brain injury; in the supplementary Erythrocyte membrane calcium Ca 2 + -Mg 2 + -ATPase activity and red blood cell Ca 2 + concentration with and without added calcium group was not statistically significant (Pgt; 0.05), free calcium concentration in plasma calcium group than those without significantly increased (P lt;0.05). Conclusion RBC HIE calcium overload, decreased plasma free calcium, and red blood cell