Neutral Cyclodextrin Capillary Electrophoresis Chiral Separation of ofloxacin enantiomers in contrast.doc

 PAGE \* MERGEFORMAT 12 Neutral Cyclodextrin Capillary Electrophoresis Chiral Separation of ofloxacin enantiomers in contrast Authors: Zhao, Xing-Bin Yang, Li Ye, WANG Qiao-Feng, ZHANG Sheng-Yong [Keywords:] Neutral Comparative study of chiral separation of ofloxacin enantiomer by capillary electrophoresis using neutral cyclodextrins [Abstract] AIM: To compare the separation ability of different neutral cyclodextrins (CD) and their derivatives on ofloxacin enantiomer by capillary electrophoresis (CE) and to explore the possible mechanisms of chiral separation. METHODS: The effects of concentration and type of neutral CD , buffer pH, running voltage and capillary temperature on the enantioseparation were investigated by CE using α CD, β CD, γ CD, hydropropylβ CD (HPβ CD) and dimethylβ CD (DMβ CD) respectively. RESULTS: Under the optimized CE conditions (50 mmol / L phosphate buffer at pH = 3.0, capillary temperature 25 ℃ , separation voltage 15 kV using 25 mmol / L DMβ CD or by the combination of 50 mmol / L HPβ CD and 25 mmol / L γ CD), ofloxacin enantiomer was completely separated with resolution as high as 2.0 and 1.7 respectively . CONCLUSION: Inclusioncomplexation mechanism is based on the cavity size and function of the rim of CDs. The proposed method was simple and rapid with high separation efficiency and can be used for enantioseparation of ofloxacin drug. [Keywords] electrophoresis, capillary; ofloxacin; enantiomer; chiral separation [Abstract] Objective: To compare the different neutral cyclodextrin capillary electrophoresis (CD) and its derivatives ofloxacin enantiomers of chiral capabilities, and to propose possible mechanisms for chiral recognition. Methods: to α CD, β CD, γ CD, HPβ CD, and DMβ CD as the chiral selector, using capillary electrophoresis study of CD type and concentration, separation voltage and temperature on separation of ofloxacin enantiomers. The results: At 50 mmol / L phosphate buffer (pH 3.0), 15 kV separation voltage an