Newborn mouse spermatogonial stem cells sperm cells.doc

 PAGE \* MERGEFORMAT 14 Newborn mouse spermatogonial stem cells sperm cells Of: Bicong Ming, Wang Shen, Wang, Fei Dong Liang, Xiao Yinxia [Abstract] Objective To investigate the mouse spermatogonial stem cells differentiate sperm cells is feasible. Methods 7 to 8 days old mouse testis as the material, the use of mouse Sertoli cells as feeder layer, application retinoic acid-induced, adult small medium induced mouse testis, and low temperature induced differentiation of spermatogonial stem cells, flow cytometry analysis of sperm-like cells induced by ploidy. by microinjection of sperm-like cells were injected into enucleated oocytes and oocytes, fertilization observed egg development situation. the result of sperm-like cells induced by three methods respectively 5.9%, 1.6%, 0.35%, flow cytometry analysis of retinoic acid ratio was 19.3% haploid, sperm cells can induce activation of oocytes , 20.36% blastocyst rate. Conclusion newborn mouse spermatogonial stem cells can be induced to differentiate into sperm cells, and has the ability to activate oocytes. [Keywords:] haploid spermatogonial stem cells in mice Abstract: Objective To discuss the possibility of SSCs of Newly-born mice inducing the differentiating Sperm-like cells. Methods Taking the testis of male mice of 7 ~ 8 days for material and adopting the testis of mice, SSCs differentiating were induced by using retinoic acid, tissue fluid of adult mice and low temperature. Flow cytometry was used to analyse the ploidy of chromosome inducing sperm-like cells. Microinjection was used to inject the sperm-like cells into oocytes and enucleate oocytes to observe the development situation of zygotes. Results The proportions of sperm-like cells induced by the three methods were 5.9%, 1.6%, 0.35% respectively. The rate of Flow cytometry to analyse retinoic acid haploid is 19.3%. The sperm-like cells achieved could activate bovine oocytes by sperm injection (ICSI), and the development rate of blastosphere