Nickel chloride on rabbit sinoatrial node cells the impact of self-discipline.doc

 PAGE \* MERGEFORMAT 13 Nickel chloride on rabbit sinoatrial node cells the impact of self-discipline Author: Shen Jian-Xin Ming Zhang Lina Zhang Xiaoyun 【Abstract】 Objective: To investigate self-nickel chloride on rabbit sinoatrial node cells. Methods: Using intracellular microelectrode recording technique to observe the 20 cases of action potential of rabbit sinoatrial node cells, as well as nickel chloride on the action potential of sinoatrial node cells impact. Results: 180μmol / L NiCl2 self sinoatrial node cells after perfusion with VDD, APA, Vmax, RPF decreased significantly (P lt;0.01), APD, APD90 was significantly prolonged, MDP, APD50 were no significant changes (Pgt; 0.05). Conclusion: The self-nickel chloride on the sinoatrial node cell action potential 0-phase and four quite different pole ion significantly inhibited. Keywords: nickel chloride; sinus; Electrophysiology The Influence of NiCl2 on Spontaneous Cells of Isolated Sinoatrial Node of Rabbit node. Methods: The characteristics of intracellular action potential were recorded from sinoartial node of 20 rabbits with mircoelectrode technique, and also that of sinoartial node perfused with 180μmol / L NiCl2 were recorded. Results: After perfused with NiCl2, The VDD, APA, Vmax , RPF obviously decreased (P lt;0.01) and the APD, APD90 were significant prolonged (Pgt; 0.05) in pacemaker cells of sinoartial node.Conclusion: It appears that NiCl2 has notably inhibitive action on the ion beam taken place in the depolarization of phase 0 and phase 4 of pacemaker cells in sinoartial node. 【KEY WORDS】 Niekel chloride; Sinoatrial node; Electrical physiology With the rapid development of electrophysiological techniques, the myocardial cells more and more in-depth study of ion channels. Ca2-channel on a wide range exists in the cell membrane, Ca2 There are many types of channels, such as voltage-gated channel and receptor-gated channels, of which the voltage-depend