Non-staining microscopic examination of urinary sediment in children with hematuria positioning of the application.doc

 PAGE \* MERGEFORMAT 5 Non-staining microscopic examination of urinary sediment in children with hematuria positioning of the application Of: Wang Wei, Feng Jia-dimensional hydrogen Liang Lanqing [Keywords:] non-renal hematuria renal hematuria Children with persistent microscopic hematuria or gross hematuria, more common in clinical, research the cause of many, often difficult to determine the moment. Hematuria positioning of the diagnosis of renal disease is important. I subjects using non-stained urine sediment microscopic examination Identification of red cell morphology, in order to distinguish glomerular hematuria and non-glomerular hematuria, and achieved better clinical results. 1 Subjects and methods 1.1 Objects and instruments The subjects in our hospital in May 2009 ~ April 2010 1000 cases of hematuria in children with pediatric clinic, 486 cases male, female 514 cases, aged 3 months to 12 years, an average of 3 years. Instrument: microscope (made in Japan OLYWUS CH -20 type) and blood cell pool (domestic). 1.2 Methods (1) take a fresh morning urine 10ml, graduated centrifuge tube placement (in polymer tube is appropriate), with turning radius of 15cm of the horizontal centrifuge 1000r/min, 5min. (2) to be stopped after the centrifuge natural Remove the centrifuge tube, remove supernatant 9.5ml, 0.5ml sediment left behind. (3) mixing tube bottom sediment, with a straw suck out 20l, trickle-down counter pool, covered with a 20mm 20mm coverslip after the examination. (4) first with 10 10 low-magnification observation of the visible picture elements, and then high magnification, 10 40, observe the red blood cell morphology. number five large boxes of red blood cell count (normal: 0.8 ~ 10,000 / ml) [1 ]. 2 Results Detection of 1000 cases of hematuria in children, men accounted for 48.6% of cases of hematuria, 51.4% female. In which red blood cell morphology was pleomorphic type 865 cases, accounting for 86.5%, red blood c