Non-viral vector gene transfer technology status and prospects.doc

 PAGE \* MERGEFORMAT 15 Non-viral vector gene transfer technology status and prospects Author: Hong-Yun Liu, Bao Yong-fen, Wang Qiang, Yao flags [Keywords:] non-viral vector gene transfer technology status and prospects Currently gene therapy has become the treatment of a variety of intractable diseases, scientists have a new means of gene therapy, gene transfer technology is also the core basic technologies. More current research into gene technology, is divided into two main categories: First, viral vector gene transfer method; 2, non-viral vector gene transfer method. The former transfection efficiency, but there are safety and immunogenicity issues. Thus, in recent years, awareness of non-viral gene delivery systems for class more attention. Non-viral vector gene transfer method is divided into physical methods and chemical methods. Physical methods such as: injection, particle bombardment, electroporation method, ultrasonic method are the use of physical force, etc. penetrate the cell membrane to achieve gene transfer; chemical methods is the use of natural or synthetic compounds that support the completion of gene transfer. Although in recent years in the field of non-viral gene transfer has achieved remarkable success, but overall, non-viral vectors relative to the viral vector is less efficient gene transfer in vivo gene transfer in particular. Now more commonly used in the current non-viral vector gene transfer method of the advantages and limitations are summarized below. A physical method Is based on the physical forces, the moment the cell membrane defects, so that plasmid DNA into the cell method. Such as particle bombardment, electroporation method, ultrasonic method, etc., as well as discovered in recent years laser-related supporting facilities. 1.1 injection Plasmid DNA directly injected into the tissue cells to achieve gene transfer purposes. Some scholars have successfully naked plasmid DNA injected into muscle, liver [1], skin [2] an