Noise exposure in guinea pig cochlea cfos gene expression in.doc

 PAGE \* MERGEFORMAT 3 Noise exposure in guinea pig cochlea cfos gene expression in Author: Zha Ding-jun, Xue Tao, Li Qiao, Liu Shun, high snow, Chen Jun, Qiu [Abstract] Objective: To observe the guinea pig cochlea after noise exposure c  fos gene expression changes. Methods: 32 healthy male white guinea pigs were randomly divided into four groups, one group of normal control group, the other three groups for the experimental group . 120 dB spL the experimental group of 4 kHz narrow band noise exposure to 4 h, were tested after noise exposure 2,48, 168 h and a control group of guinea pig auditory brainstem response (ABR). immunohistochemical staining analysis of c  fos gene expression in the cochlea, using computer image analysis system for measurement of c  fos protein in the cochlea, the average absorbance values. Results: guinea pigs after noise exposure ABR threshold and c  fos protein in the cochlea, the average absorbance value higher than the normal group were increased; over time, the extension of ABR threshold and c  fos protein in the average absorbance values are gradually restored, 168 h group of ABR threshold and c  fos protein in the average absorbance values are less than 2 h group and the 48 h group. Conclusion: the cochlea after noise exposure increased c  fos gene expression may be related to cochlear hair cells after noise exposure, spiral ganglion structure in repairing the damage. [Keywords:] c  fos gene; cochlea; noise; guinea pigs [Abstract] AIM: To investigate the changes of c  fos expression in the cochlea of guinea pigs after noise exposure. METHODS: Thirty  two healthy male guinea pigs were randomly distributed into 4 groups: a normal control group, and 3 experimental groups. The experimental groups were exposed to 4 kHz narrow band noise at 120 dB spL for 4 h so as to induce the cochlea damaged animal model. Auditory brainstem response (ABR) was test before and 2, 48, 168 h after noise exposure. Immunocytoch