Nitidine chloride induced apoptosis of hepatoma cells SMMC7721 Study.docVIP

 PAGE \* MERGEFORMAT 13 Nitidine chloride induced apoptosis of hepatoma cells SMMC7721 Study Of: Li Danni, Liu Hua steel, Liu Limin, Liu Lin, Liang cream [Abstract] Objective nitidine chloride induced hepatocellular carcinoma SMMC 7721 cells apoptosis. Methods thiazolyl blue (MTT) colorimetric assay SMMC 7721 human hepatoma cell growth activity, Hoechst33258 nuclear staining, cell morphology was observed changes, flow cytometry cell cycle and apoptosis. Results nitidine chloride effect on cells in vitro in a concentration and time dependent manner significantly inhibited the growth of SMMC 7721 cells, the role of half 24,48,72 h inhibitory concentration (IC50) were (19.5 + -1.8), (3.8 + -0.2), (2.8 + -0.1) mg / L, Hoechst33258 staining, showing nuclear chromatin condensation, apoptotic cells showed a dense concentration of dye, and the control group, nitidine chloride treatment increased the proportion of apoptotic cells by flow cytometry to the G2 / M cell ratio increased, G0/G1, S phase cells decreased. drug effects on SMMC 7721 cells, with concentration increased apoptosis rate increased. Conclusion nitidine chloride on SMMC 7721 cells significantly inhibited the growth may be related to changes in the cell cycle and induce apoptosis. [Keywords:] nitidine chloride, SMMC 7721 cells, G2 / M phase arrest, apoptosis, liver ABSTRACT: Objective To study the effect of nitidine chloride in inducing apoptosis of human hepatoma cell SMMC 772l. Methods Cell viability was measured by MTT assay in human hepatoma cell line SMMC 7721. Morphological changes of cell nuclei were observed by Hoechst 33258 staining. Flow cytometry was performed to measure the cell cycle and cell apoptosis. Results Proliferation of SMMC 7721 cells was significantly inhibited by nitidine chloride in concentration and time dependent manners in vitro, the 50% inhibition concentration (IC50) value at 24, 48, and 72h being ( 19.5 + -1.8), (3.8 + -0.2) and (2.8 + -0.1) mg / L, respectively