Norketamine hapten and its wholly-antigen synthesis and identification of.doc

 PAGE \* MERGEFORMAT 11 Norketamine hapten and its wholly-antigen synthesis and identification of Of: YANG Ting-ting Zhao Shen Xiaopingtanbo City Zoupan Kun Chang DEVELOPMENTS Liu Lingyun Wei Chao Wei Zhao Wanli Jinhai Wu Jie Zhang Dongsheng United [Abstract] at low temperature to a ketamine and succinic acid was synthesized hapten carboxyl - norketamine, electrospray ionization mass spectrometry results show that the target hapten was synthesized successfully by the carbodiimide method hapten carrier protein coupled with the preparation of artificial antigens, identification of infrared spectroscopy results showed that the success of artificial antigen synthesis, matrix-assisted laser desorption ionization time of flight mass spectrometry showed that norketamine hapten conjugated to bovine serum albumin ratio of 11:1. The animal immunization, access to high titer specific polyclonal antibody titer up to 5.12 × 104. [Keywords:] norketamine, hapten, artificial antigen, synthesis, identification Synthesis and Identification of Hapten and Complete Antigen of Norketa mineYANG Ting-Ting, ZHAO Chun-Cheng, SHEN Xiao-Ping, TAN Bo-Chang, ZOU Pan-Kun, WANG Huai-Ming, LIU Ling-Yun, WEI Chao, WEI Wan -Li, HE Jin-Hai, WU Jie, ZHANG Dong-Sheng, ZHAO Xiao-Lian (Jiangsu SUWEI Microbiology Research Co., LTD., Wuxi 214063) (Wuxi Municipal Public Security Bureau, Wuxi 214002) (Zhenjiang Municipal public Security Bureau , Zhenjiang 212001) (Jiangyin Municipal public Security Bureau, Jiangyin 214405) Yancheng Municipal public Security Bureau, Yancheng 224001) Abstract Under low temperature conditions, the hapten carboxyl-norketamine was synthesized by reacting norketamine and succinaldehyde acid.Identification result using electrospray ionization mass showed the hapten was successfully synthesized.The artificial antigen confirmed by infrared spectroscopy was developed by conjugating hapten to carrier proteins with carbodiimide (EDC) method.Matrix-assisted laser desor