NOX4 reporter gene plasmid and expression regulation of.doc

 PAGE \* MERGEFORMAT 11 NOX4 reporter gene plasmid and expression regulation of On: Jun Zhao Liu Yanxiang Liu Zhen Wang Boyao Huang Ning Wuqi [Abstract] Objective: To construct NOX4 luciferase reporter gene plasmid vector, gene expression and regulation of NOX4. METHODS: The cell genomic DNA as a template for using PCR amplification of NOX4 gene upstream regulatory sequence (533bp), was inserted into pGL3 basic vector The recombinant plasmid pGL3 NOX4, the recombinant plasmid by enzyme digestion and sequencing. the pGL3 NOX4 transfected A549 cells by stimulating cytokine gene expression observations. Results: NOX4 enzyme digestion and sequencing confirmed that the report gene plasmid was successfully constructed. transfection reporter gene in A549 cells stimulated cytokines showed increased expression of NOX4. CONCLUSION: The constructed NOX4 luciferase reporter gene plasmid vector for further study of NOX4 gene expression pattern and physiological functions of the foundation. [Keywords:] NOX4; reporter gene; expression regulation The reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase first found in neutrophils and monocytes / macrophages, phagocytosis of microbial pathogens at the time, NADPH oxidase is activated, the cell respiratory burst, resulting in a large number of ROS (reactive oxygen species, ROS), as an important body composition against microbial infections [1]. In recent years, the plasma membrane in non-phagocytic NADPH oxidase found in the family homolog, named NOX (NADPH oxidase) protein family. the human genome homology search found 7 kinds of NADPH oxidase, that NOX1, NOX2, NOX3, NOX4, NOX5, Duox1 and Duox2 [2-4] .. NOX family of widely expressed in body tissues and organs, their biological function as a hot area of #8203;#8203;current research. In order to investigate the NOX family of NOX4 in the inflammatory and immune responses in the role, we constructed reporter gene plasmid NOX4 pGL3 NOX4, and we observed th