Nucleated cells in septic rats PPAR activity on TNF- and IL-4 of.doc

 PAGE \* MERGEFORMAT 20 Nucleated cells in septic rats PPAR activity on TNF- and IL-4 of Of: Huang Xiaomei, who Mian, Lu Guifang, OFF Pan, then Yellow Book [Abstract] [Objective] when sepsis rat cells peroxisome proliferator-activated receptor (PPAR) activity, plasma levels of inflammatory mediators TNF-@ and anti-inflammatory IL-4 media changes explore the relationship. [Methods] Male 90 SPF SD rats, weighing 280 ~ 300 g, were randomly divided into normal control group, sham operation group, sepsis groups, each subdivided into 12 h, 24 h, Sanya, 48 h group, n = 10. cecal ligation and puncture (CLP) rat model of sepsis, ELASA nucleated cells was determined PPAR activity and plasma levels of IL-4 in the trend. [Results] pus toxemia group plasma proinflammatory TNF-@ and the normal control group and increased significantly compared with the sham group, respectively (162 + - 32 vs 31 + - 7, 32 + - 8) pg / mL, with prolonged upward trend peaked at 24 h after surgery, then gradually decreased, but still at a high level state. antiinflammatory mediators IL-4 levels were also significantly higher than the normal control group and sham group increased (89 + - 25 vs 41 + - 7, 41 + - 7) pg / mL, and showed persistent elevated (70 + - 5, 75 + - 8, 122 + - 11) pg / mL, there is a balance of proinflammatory and anti-inflammatory disorders. sepsis group PPAR activity in nucleated cells and normal control group and significantly lower than the sham group, respectively (0.263 + - 0.017, vs 0.292 + - 0.005, 0.294 + - 0.007) and increased with disease gradually decreased. sepsis group PPAR activity in nucleated cells and plasma proinflammatory TNF-@ levels were negatively correlated, r = -0.655, P = 0.040. [Conclusion] The presence of inflammation in septic rats - anti-inflammatory system imbalance, decreased activity of PPAR nuclear cells and plasma proinflammatory TNF-@ was negative. [Keywords:] peroxisome proliferator-activated receptor; inflammatory mediators; seps