Not artificial ventilation in rat model of acute myocardial infarction.doc

 PAGE \* MERGEFORMAT 16 Not artificial ventilation in rat model of acute myocardial infarction Of: Wei Feng, Zhang, Han Ke, Liu Yu, Wang Ting Zhong, Niya Juan, Ai-Qun Ma [Abstract] Objective ventilation in non-labor conditions, less trauma and to easily and quickly establish a rat model of acute myocardial infarction. Methods 30 adult SD rats were anesthetized, the spontaneous breathing condition, the left sternal border section 4 intercostal muscle intercostal blunt dissection, exposing the heart, the rapid ligation of the left anterior descending coronary artery (LAD) model to establish myocardial infarction. also recorded before, during and after 1,3 d, 1,2 weeks of ECG changes ; 2 weeks after thoracotomy, the heart of the general morphological changes observed, LAD blood supply zone in the infarcted area was white model is the successful; while taking eight models in the heart of HE stained histological specimens to confirm the model. results of 25 rats successfully established myocardial infarction model, the model survival rate was 83.33%. electrocardiogram monitoring showed that after LAD ligation to reduce the R peak, appears ST segment elevation and ST-T fusion, 30min after the shows pathological Q waves. surgery 2 weeks after thoracotomy, myocardial ischemia can see the color white, wall motion decreases. HE staining showed disappearance of cardiac myocytes and on behalf of a large number of fibers and collagen tissue, myocardial infarction border zone residual disorder, broken filaments interstitial structural damage, fibrosis. Conclusion The method can not artificial ventilation conditions, the fast, simple and effective model of rat acute myocardial infarction. [Keywords:] not artificial ventilation; myocardial infarction; animal model; rat ABSTRACT: ObjectiveTo establish a model of acute myocardial infarction in rats without artificial ventilation.MethodsAfter anesthesia, left thoracotomy was performed in 30 adult SD rats by blunt diss