Nucleostemin gene specific RNA interference on cell proliferation and apoptosis.doc

 PAGE \* MERGEFORMAT 12 Nucleostemin gene specific RNA interference on cell proliferation and apoptosis Of: Zhengxue Zhi, Hu Jing, Wei Sun, Liu Guilian, Liu Ni, Caizai micro [Abstract] Objective To investigate the inhibition of RNA interference nucleostemin (NS) gene expression on gastric cancer SGC 7901 cell proliferation and apoptosis. Cationic liposome method to build a good NS siRNA expression vector was transfected into gastric cancer cell line SGC 7901 by MTT method testing the inhibitory rate of cell proliferation, RT PCR method to detect the NS gene expression in cells, cell cycle detected by flow cytometry, Annexin V FITC / PI apoptosis assay. Results Compared with the control group , S slowed down the proliferation of cells, 24,48,72 h cell proliferation inhibition rate was 53.21%, 71.54%, 87.47%, NS decreased gene expression, G0/G1 phase cell percentage was significantly higher (58.34%), S phase cells decreased (20.68%), apoptosis rate increased (26.85%). Conclusion RNA interference can inhibit gastric cancer cell line SGC 7901 NS genes, the cell proliferation slowed down, cell cycle arrest in G0/G1 phase and ends death rate increased. [Keywords:] RNA interference, nucleostemin, gastric cancer, cell proliferation, apoptosis ABSTRACT: Objective To investigate the effect on proliferation and apoptosis by RNA interference to inhibit Nucleostemin (NS) gene expression in gastric carcinoma SGC 7901 cells. Methods The NS siRNA expression vector was transfected into gastric carcinoma cells with LipofectamineTM2000 reagent. Then we detected the cell proliferation inhibition ratio by MTT assay, the levels of NS gene expression in all groups by RT PCR, cell cycle by flow cytometry, cell apoptosis ratio by Annexin V FITC / PI kit. Results Compared with that in the control group, cell proliferation in S group decreased; at 24, 48 and 72h the cell proliferation inhibition ratio was 53.21%, 71.54% and 87.47%, respectively, the level of NS gene expressio