Nutgall alcohol extract of Pseudomonas aeruginosa biofilms of Bacillus.doc

 PAGE \* MERGEFORMAT 12 Nutgall alcohol extract of Pseudomonas aeruginosa biofilms of Bacillus Author: Huang Xiaomin, Jane Run well, Yu-ling, had ear red, Jane laughed Huan, Mr Wong, Chung-Hsing Wu [Abstract] Objective To study the gallic alcohol extract of Pseudomonas aeruginosa biofilm bacteria effects. Methods for dilution method in vitro determination of gallic alcohol extract of Pseudomonas aeruginosa bacteria minimum inhibitory concentration (Minimal Inhibitory Concentration, MIC). Replication in vitro using flat-panel modified bacterium Pseudomonas aeruginosa biofilm model, and to detect alcohol extract of different gallic MIC concentration of Pseudomonas aeruginosa biofilm bacteria effect. Results nutgall alcohol extract of Pseudomonas aeruginosa bacilli MIC of 31.25 mg / ml, 1.5 times the MIC (46.8 mg / ml) can significantly reduce the number of viable biofilm, but not completely removed bio - membrane of bacteria, two times the MIC (62.5 mg / ml) on the role of bacterial biofilm are completely removed. Conclusion nutgall on Pseudomonas aeruginosa biofilm scavenging effect, but it needs to conduct clinical drug research. [Keywords:] gallnut alcohol extract of Pseudomonas aeruginosa biofilm bacteria Bacillus Abstract: ObjectiveTo study the effects of ethanol-extract of G.chinensis on P. aeruginosa Biofilm. MethodsMinimal inhibitory concentration (MIC) was tested by double dilution, and P. aeruginosa biofilm was induced in vitro by improved Brownplate method. The effects of ethanol-extract of G. Chinensis in different MIC on P. aeruginosa biofilm were determined. ResultsThe MIC of ethanol-extract of G. chinensis to P. aeruginosa was 31.25mg/ml, 1.5MIC (46.8mg/ml) of Galla Chinensis decoction coulde obviously reduce the amount of bacteria on P. aeruginosa biofilm, and 2MIC (62.5 mg / ml) had the eliminating effect on it. ConclusionGalla Chinensis has the eliminating effect on P. aeruginosa Biofilm, which still need clinical research. Keywords:: Ethan