Of sodium selenite on K562-ADR reversal of multidrug resistance and its mechanism.docVIP

 PAGE \* MERGEFORMAT 11 Of sodium selenite on K562/ADR reversal of multidrug resistance and its mechanism Author: Cui Jing, Ding Jing, WU Yi-ping, Liu Fu-Qiang, Liu Chao, Wang Yang Abstract In this study of sodium selenite on drug-resistant leukemia cell lines K562/ADR cell reversal of multidrug resistance and its reversal of the mechanism. With methyl thiazolyl tetrazolium (MTT) assay K562/ADR sodium selenite on cell growth inhibition and its impact on K562/ADR cells, the role reversal of drug resistance; application of flow cytometry of sodium selenite on K562 and K562/ADR apoptosis rate impact; using semi-quantitative RT  PCR assay K562/ADR cells mdr1 and bcl  2 gene expression. The results showed that, 10 μmol / L sodium selenite can significantly increase the sensitivity of K562/ADR cells to doxorubicin and its resistance reversal multiple of 2.31; early apoptosis rate of 10 μmol / L sodium selenite effect on K562 cells, 48 hours is elevated; while middle and late apoptotic rate of sodium selenite 5,10 μmol / L, when the role of 48,72 hours were significantly higher; two kinds of concentrations of sodium selenite for 48 hours in the role of the K562 can / ADR cells in early apoptosis rate increased, the role of 48,72 hours can K562/ADR cells and late apoptosis rate increased, 10 μmol / L of sodium selenite induced apoptosis rate is higher than 5 μmol / L, for 72 hours of apoptosis rate is higher than 48 hours; sodium selenite can K562/ADR cells mdr1 mRNA and decreased expression of bcl  2 mRNA. Conclusion: The sodium selenite can be partially reversed K562/ADR cell resistance, its mechanism may be to increase K562/ADR apoptosis rate, reduced mdr1 mRNA and bcl  2 mRNA expression. Keywords: Apoptosis Reversal Effect of Sodium Selenite on Multidrug Resistance in K562/ADR Cell Line and Its Mechanisms Abstract This study was purposed to investigate the reversal effect of sodium selenite on multidrug resistance in adriamyc