Of soy isoflavones on hydrogen peroxide-induced intracellular calcium overload.docVIP

 PAGE \* MERGEFORMAT 12 Of soy isoflavones on hydrogen peroxide-induced intracellular calcium overload Of: Greate Zhang Yuwei Zhang Wenjie Chun-Yan Zhao [Abstract] Objective To observe the soybean isoflavones (SI) on H2O2-induced primary cultured neonatal rat myocardial cells the antagonism of calcium overload. Methods Wistar vitro neonatal rat myocardial cell culture, the cardiomyocytes were divided into control group , H2O2, and H2O2 injury model group SI and injury treatment group: SI 0.1,1.0,10 g / ml dose, pre-used after 24 h to give SI 0.3 mmol / L H2O2 injury of myocardial cells to load fluorescent indicator Fluo 3/AM , confocal laser microscopy, respectively, immediately after adding H2O2 and 15 min, detection (Ca2 +) i. Results can be seen 15 min dynamic monitoring of myocardial cells in control group the average fluorescence intensity low; H2O2 added immediately after cells fluorescence began to increase density, 15 min and the fluorescence intensity of intracellular fluorescence intensity was significantly higher (P lt;0.01); H2O2 injury plus SI treatment groups: pre-to the SI 0.1,1.0,10 g / ml three dose group (Ca2 +) i resting fluorescence intensity values, and calcium homeostasis of the peak value of fluorescence intensity compared with H2O2 injury group decreased significantly (P lt;0.01); also seen compared with the control group, three levels of SI can be made intracellular calcium ((Ca2 +) i) concentration of the average fluorescence intensity was significantly lower (P lt;0.01). Conclusions SI could significantly reduce the H2O2-induced cultured cardiac cells Ca2 + overload, and with the drug concentration gradually increased , fluorescence intensity of intracellular calcium but decreased in a dose dependent manner. [Keywords:] soy isoflavones; cultured myocardial cells; calcium; hydrogen peroxide [Abstract] Objective To examine the effects of hydrogen peroxide on intracellular free calcium concentration ((Ca2 +) i) in cardiomyocy