Of high glucose on human umbilical vein endothelial cells GSH.PX activity NOS expression and ICAM.1.docVIP

 PAGE \* MERGEFORMAT 3 Of high glucose on human umbilical vein endothelial cells GSH.PX activity NOS expression and ICAM.1 Authors: ZENG Yu, Deng, Wang Jun, Su Ning, Liu Dongfeng [Abstract] Objective: To investigate the in vitro culture conditions, the state high glucose on vascular endothelial cells. Methods: The cultured human umbilical vein endothelial cell line ECV304, were divided into control group and the high glucose group, using spectrophotometric assay for measuring glutathione peroxidase (GSH.PX) activity, RT.PCR assay cell adhesive adhesion molecule-1 (ICAM.1) mRNA levels were observed by immunocytochemistry endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) protein expression. Results: High glucose group GSH.PX activity decreased, eNOS, iNOS protein expression was significantly increased, ICAM.1mRNA levels have also increased significantly. Conclusion: The elevated glucose concentration in a relatively short period of time can cause vascular endothelial cells GSH.PX activity decreased, ICAM.1mRNA, eNOS and iNOS protein expression increased in the early progress of diabetic vascular disease play a role. [Keywords:] high-sugar; human umbilical vein endothelial cell; nitric oxide synthase; intercellular adhesion molecule-1 Diabetic vascular complications are leading causes of death in patients with diabetes, one of the vascular endothelial cell injury is the foundation. Chronic hyperglycemia and vascular complications in the relationship is very positive, but short-term, acute hyperglycemia on vascular lesions affecting little research. This study was the short-term high glucose stimulation on cultured human umbilical vein endothelial cell line ECV304 biological characteristics, in order to further explore the early progress of diabetic vascular disease provide some experimental basis for the mechanism. 1 Materials and methods 1.1 cells, reagents and equipment ECV304 were purchased from Chinese Academy of S