On lipid-lowering diet Determination of puerarin content of particles.docVIP

On lipid-lowering diet Determination of puerarin content of particles [Abstract] Objective Determination of puerarin in lipid-lowering diet grain content. Methods Shim-pack-C18 column (5 μm, 150 mm × 4.6 mm), methanol -0.1% phosphoric acid (25.3:74.7) as the mobile phase, flow rate 0.9 mL / min, detection wavelength was 250 nm. The results of the linear range of 0.114 ~ 0.570 μg, r = 0.999 6, recovery was 98.74%, RDS = 0.67%. Conclusion The method is simple, accurate, sensitive and can be used for the preparation of quality control. [Key Words] high performance liquid chromatography, puerarin, lipid-lowering diet particles Abstract: Objective To determine the content of Puerarin in Jiangzhijianfei Granules by high performance liquid chromatography. Methods The determination was conducted by HPLC using a Shim-pack-C18 column (5 μm, 150 mm × 4.6 mm) and a mobile phase of methanol- 0.1% Phosphoric acid water (25.3:74.7). The flow rate was 0.9 mL / min and the detection wavelength was 250 nm. Results Good linear relationship between area and amount was noted for 0.114 ~ 0.570 μg of Puerarin, with a correlation coefficient of 0.999 6. The average recovery of was 98.74%, RSD was 0.67%. Conclusion The established method is simple, accurate, sensitive and can be applied to the quality control of the preparation. Keywords:: HPLC, Puerarin; Jiangzhijianfei Granules Particles by lipid-lowering diet multiflorum, Kudzu, Panax herbs such as 10 formed with a nourishing liver and kidney, raising essence and blood, strengthening and restoring, meridians and pain, spleen Phlegm, eyesight Sheng Jin, laxative efficacy for various types of hyperlipidemia, cardiovascular and cerebrovascular sclerosis, obesity, habitual constipation, bleeding hemorrhoids. Fang Kudzu as the main drug. In this study, on the preparations by HPLC to determine the content of puerarin in order to better control the quality of their products. An instrument and reagent Shimadzu LC-10