On magnesium and clinical significance of the detection method.docVIP

 PAGE \* MERGEFORMAT 11 On magnesium and clinical significance of the detection method [Abstract] Magnesium is the body of one of the most abundant cations. Atomic formula Mg, atomic weight 24.31. Adult body magnesium 0.823 ~ 1.234 mol, 50% of which exist in the bone, 45% in intracellular fluid, extracellular fluid accounted for 5 %. liver, kidney and muscle magnesium are more liquid in the cell just below the potassium content of magnesium in door No. 2, its concentration is about 10 times the extracellular fluid. in the extracellular fluid, second only to the content of magnesium in sodium, potassium, calcium door to the fourth. [Keywords:] cells tested in liquid magnesium Chemical processes in many physiological responses and magnesium are involved in an important role. Such is the variety of enzyme activator, alkaline and acid phosphatase, phosphate mutase, pyrophosphate enzyme, creatine kinase, hexokinase, leucyl aminopeptidase and acid carboxylase, etc., they are required to have catalytic activation of magnesium, magnesium is also composed of DNA, RNA and ribosomal elements necessary for macromolecular structure, but also maintain normal nerve function and muscle of the important elements. Magnesium by atomic absorption spectrophotometry, is the use of magnesium in the 285.2 nm has a strong line emission or absorption, making it easy to be separated, especially suitable for the determination of the concentration of magnesium in biological fluids, is the reference method for the determination of magnesium, are widely used. The direct use of a dye spectrophotometry, the accuracy and precision can be achieved clinical requirements, and suitable automatic analysis, widely used in clinical laboratories. 1-methyl thymol blue method 1.1 Principles of serum magnesium and calcium ions in alkaline solution with methyl thymol blue dye-binding, generating a blue complex, can be masked by adding EGTA calcium interference. With the sa