On Orthogonal extraction Xiaochuan Plaster.docVIP

 PAGE \* MERGEFORMAT 7 On Orthogonal extraction Xiaochuan Plaster [Abstract] Objective Optimization Xiaochuan Plaster the best extraction. Method using high performance liquid chromatography to determine the main component of prescription Angelica imperatorin content, with the L9 (34) Orthogonal Design of the ethanol extract good condition. The best results Xiaochuan Plaster extraction process as follows: 90% ethanol concentration, solvent amount was 2 times amount of reflux time 1 h, 2 times reflow. Conclusion The method is stable and extraction rate. [Keywords:] Xiaochuan Plaster, imperatorin, high performance liquid chromatography, orthogonal design Abstract: Objective To optimize the preparation process of Xiaochuan Tiegao. Methods The effective compound imporatorin of Dahurian Angelica Root was determined by HPLC. The best condition of preparation process for the ethanol-extraction method was investigated by L9 (34) orthogonal design. Results The optimum technical condition: adding 2 times ethanol, cooking 1 hour and 2 times with 90% ethanol. Conclusion The method is reliable, accurate and easy to operate. Keywords:: Xiaochuan Tiegao, imporatorin, HPLC, orthogonal design Xiaochuan Plaster is Angelica, Mustard Seed, Asarum, fumarate made of 6 herbs Chinese herb compound, with cough, phlegm, asthma features, mainly for chronic bronchitis, bronchial asthma. Side in Angelica King and medicine, and more prescriptions, the active ingredient imperatorin has antibacterial, anti-allergic asthma, and so on. In order to play the best drug efficacy and economic efficiency, optimize the preparation process, before the trial to Europe Hu pigment content as an indicator Orthogonal with the best extraction conditions, provide the basis for industrial production and extraction. 1 apparatus and materials Alliance 2690 HPLC (U.S. Waters), PDA-2996 diode array detector (U.S. Waters), Empower Data Processing System (U.S. Waters). Imperatorin st