On the atomic force microscope in bio-medical applications.docVIP

 PAGE \* MERGEFORMAT 22 On the atomic force microscope in bio-medical applications Abstract: In this paper, the working principle of atomic force microscope, atomic force microscope applications, AFM study of progress in the chromosome and looking forward to the four aspects of the brief. Keywords:: Bio-Medical Applications of atomic force microscope Working principle of an atomic force microscope Atomic force microscopy (Atomic force microscopy, AFM) is a kind of physics based on the sample surface by scanning probe for imaging the interaction between atoms of new surface analysis instrument. It belongs to following the optical microscope, electron microscope after the third-generation microscope. Often use a very sharp AFM probe scans the sample, the probe fixed to the probe and the sample surface forces on the highly sensitive micro-cantilever. Cantilever deflection will cause the force sent by the laser source laser beam reflected by the cantilever after the shift. Detector receiving the reflected light, finally accept the signal through the computer system acquisition, processing, forming the sample surface topography images. Early development for contact atomic force microscope, which includes the constant force mode and Constant-height mode. The former displacement caused by the use of reflected light photodiode output voltage feedback loop constitute a change in control of piezoelectric ceramic tubes with telescopic, fixed on the scanner to adjust the location of the sample, maintaining force between the sample and probe (cantilever bending) the same measuring the changes in height of each point. The latter to maintain the distance between the sample and probe change, measuring the size of each point force. This pattern in the regulation of the probe and the sample can be directly observed from the previous cantilever curvature changes. In addition to the traditional contact-type addition, in 1993 they developed a tapping-type a