On the LC-MS-MS in the identification of health food ingredients in the application of theophylline.docVIP

 PAGE \* MERGEFORMAT 7 On the LC-MS-MS in the identification of health food ingredients in the application of theophylline [Abstract] the development of health food illegally added drugs - theophylline qualitative and quantitative components. This method main parameters: The column was Agilent KromasilC18 (150mmX4.6mm, 5μm), mobile phase of methanol water (30:70) detection wavelength of 270nm, flow rate was 1ml · min-1 (liquid) flow rate of 0.3ml · min-1 (MS); this method, the average recovery of theophylline was 99.52%, RSD was 0.81% (n = 5). The method is simple, accurate, specific, and can be used for health food ingredients illegally added drugs theophylline determination and identification. [Key Words] high performance liquid chromatography mass spectrometry; health care products; theophylline HPLC-MS is the high separation capacity, the use of a very wide range of liquid chromatography separation techniques (including capillary electrophoresis, capillary high performance liquid chromatography) with high sensitivity, high-specific mass spectrometry combined into a kinds of powerful, multi-purpose qualitative and quantitative analysis tools. The high performance liquid chromatography mass spectrometry, the illegal add on health care drugs theophylline determination of quantitative and qualitative. Confirmed the molecular ion peak is mode 181, the negative mode for the 179. The experimental results show that the method is simple , rapid, specific, and can be used for qualitative and quantitative determination of theophylline. An instrument and reagent LC 1200 High Performance Liquid Chromatography (Agilent production), VWD UV detector, Agilent ChemStation, 6300 mass detector (Agilent Technologies company), electrospray ionization source (ESI source), Pulisaisi Electronics analytical balance. theophylline standard (batch number: 100 121 199 903, Pharmaceutical and Biological Products), a spirit of two groups of asthma (× × × City Hi-tech bio