On the fluvastatin induced differentiation of HL60 cells inhibited the expression of VEGF mRNA.docVIP

 PAGE \* MERGEFORMAT 11 On the fluvastatin induced differentiation of HL60 cells inhibited the expression of VEGF mRNA Of: Zhao Liyan Miao Lin Chun-sheng Li Shi Yan Yun Qian [Abstract] Objective fluvastatin (fluvastatin, Flu on induced differentiation of HL 60 human promyelocytic leukemia cells (HL 60 cell vascular endothelial growth factor (VEGF) mRNA expression of the inhibitory effect and its mechanism. Methods in vitro HL 60 cells were randomly divided into control group, positive control group, Flu treatment group and Flu + mevalonate (Mev treatment. Flu Flu + Mev treatment group and treatment group by adding a final concentration of 20 mol / L of the Flu, Flu + Mev Flu treatment group, while adding the final concentration Mev 100 mol / L, positive control group, the final concentration ATRA 10 mol / L, the control group an equal volume dimethyl sulfoxide (DMSO, 72 h, cells were harvested after. Cells detected after 72 h treatment NBT reduction capacity, application of RT PCR to detect the VEGF mRNA expression in HL 60 cells. Results Flu NBT reduction ability treated cells (0.63 + -0.09 compared with the control group (0.22 + -0.06 was significantly higher (P lt; 0.01, Flu + Mev NBT reduction ability of treated cells decreased significantly (0.34 + -0.04 (P lt;0.01, showed that Flu had been induced HL 60 cell differentiation, and can be reversed by adding mevalonate Flu induced differentiation. positive control group and the Flu VEGF mRNA expression levels of treatment were 1.51 + -0.02 and 1.58 + -0.04, compared with the control group (1.87 + -0.05 was significantly lower (P lt;0.01, Flu + Mev treatment reversed the expression of VEGF mRNA induced by the Flu downward (1.76 + -0.06, and Flu treatment group was statistically significant (P lt;0.05. Conclusion differentiated Flu can inhibit VEGF gene expression in HL 60 cells, which may be through the “mevalonate pathway” mediated. [Keywords:] Fluvastatin, HL 60 cells, vascular endothelial growth fa