Paclitaxel and gemcitabine on nasopharyngeal carcinoma cell line HNE2 synergistic role in radiosensitization.docVIP

 PAGE \* MERGEFORMAT 10 Paclitaxel and gemcitabine on nasopharyngeal carcinoma cell line HNE2 synergistic role in radiosensitization 【Abstract】 Objective To observe the paclitaxel and gemcitabine two-drug combination of nasopharyngeal carcinoma cell line HNE2 the radiation sensitizing effect, and to explore its possible mechanism. Methods HNE2 cells were divided into irradiated group, gemcitabine (GE) + irradiation group, paclitaxel (PA) + irradiation group and GE PA irradiation group, using colony forming assay calculate two drugs alone and combined radiation sensitizer ratio, flow were detected by cytometry to compare each group the rate of apoptosis and cell cycle. Western blot determination of each group of apoptosis-related genes bcl  2, bax expression. The results of gemcitabine, paclitaxel and the two-drug combination used in radiosensitization than 1.06,1.13 and 1.34, respectively. By flow cytometry showed Gemcitabine and radiation-induced S phase arrest of the main (96.03%), induction of apoptosis rate was 31.62%; Paclitaxel-ray induced block in G2 + M phase the majority (75.71%), induced cell apoptosis of 44.56%; two drug combination and co-irradiated G2 + M phase fraction (65.98%) decreased, while S-phase fraction (33.33%) increased (compared with the PA radiation), the apoptotic rate reached 73.28%. In the four groups in the expression of bcl  2 showed gradual downward trend, while the expression of bax was then gradually rise. Conclusion gemcitabine, paclitaxel alone and the two drugs in combination on the nasopharyngeal carcinoma cell line HNE2 have radiation sensitizing effect, and both synergistic sensitizing effect was significantly higher than the sensitizing effect alone, showing the two drugs in combination help to improve the nose pharynx cancer radiosensitivity. Taken together mechanism of radiation sensitizing effect may be related to apoptosis. Keywords: Paclitaxel Gemcitabine Radiosensitization of nasopharyngeal ca