Paederiae essential oil in vitro effect of anti-hepatitis B virus_0.docVIP

 PAGE \* MERGEFORMAT 12 Paederiae essential oil in vitro effect of anti-hepatitis B virus Of: Zhu Ning, Huang Dinan, Hou dare, Ge Qinghua [Abstract] Objective paederiae volatile oil against HBV in vitro antiviral effect of experimental study. Methods steam distillation essential oil Paederiae to HepG 2.2.15 cells as experimental objects, detecting volatile oil Paederiae hepatoma HepG 2.2.15 cell cytotoxicity. and the maximum non-toxic concentrations observed paederiae volatile oil of HepG 2.2.15 cells expressing HBsAg, HBeAg effect. Results paederiae volatile oil in the 31 ~ 2 000 mg / L in the concentration range of HepG 2.2 .15 cells was significantly inhibited, TC50 is 1 550 mg / L, TC0 to 500 mg / L. In the biggest non-toxic concentrations, volatile oil Paederiae maximum inhibition of HBsAg was 72.49%, the treatment index was 6.74, the largest of HBeAg inhibition rate was 23.64%. Conclusion paederiae HepG2.2.15 cells of volatile oil of HBsAg, HBeAg secretion in a better inhibitory effect. [Keywords:] paederiae; hepatitis B virus; HepG2.2.15 cell line; volatile oil; hepatitis B surface antigen; hepatitis B e antigen Paederiae are Rubiaceae (Rubiaceae) is Paederiae (Paederia) trailing herbaceous perennial vine paederiae Paeder scandens (Lour.) Merri. The whole plant, the sweet and sour, mild, is a commonly used Chinese herbal medicine [1] widely distributed in China, Japan, Korea, Vietnam, Thailand, the Philippines, India and the U.S. west coast. Experience shows that traditional Chinese medicine in jaundice, hepatitis, hepatosplenomegaly and other treatment has a role [2,3], as an effective anti-HBV supporting herbs in traditional Chinese medicine in a very wide compatibility; while its leaves and tender stems of Guangdong; Hainan area residents like to eat delicious vegetables [4], with high food security. In this study, in the essential oil of paederiae role of anti-HBV antiviral in vitro experimental study with a view to further development Pa