Panax notoginseng on liver tissue with obstructive jaundice bax bcl.docVIP

 PAGE \* MERGEFORMAT 9 Panax notoginseng on liver tissue with obstructive jaundice bax bcl Author: Zhou Bin, Yu Bo, Nan Yao, Song Shao-li [Keywords:] obstructive jaundice;, bax;, bcl [Abstract] AIM: To explore the protective mechanism of Panax notoginoside (PNS) against hepatic injury after obstructive jaundice (OJ) in rabbits. METHODS: Fortyeight healthy Japanese white rabbits were randomized into 2 groups, 24 in each group: group A treated with common bile duct ligation (BDL), group B of BDL PNS. All animals were kept under the same condition. Immunohistochemistry was used to determine the expressions of Bax and Bcl2 in hepatic tissues. RESULTS: In group A, expressions of bcl2 and bax in the hepatic tissue were observed after OJ. Expression of bax reached the peak at the 9th day after OJ, then tended to decline. Similarly, the highest expression of bcl2 was observed at the 21th day after OJ. The apoptosis index (AI) increased with the increase of bax expression but decreased with bcl2 strong expression. After treatment with PNS in group B, a lower expression of bax and a higher expression of bcl2 were found, and the AI value was also lower, compared with group A. CONCLUSION: The pathology of OJ may be related to the initiation of apoptosis. PNS may play a protective role against hepatic injury after OJ, which may be realized through upregulating the expression of bcl2 and downregulating the expression of bax in the hepatic tissues. [Keywords] obstructive jaundice; bax; bcl2; Panax notoginoside; liver; rabbits [Abstract] Objective: To evaluate the liver injury in obstructive jaundice pathological regulatory mechanisms, and panax notoginseng saponins (PNS) of protection. Methods: 48 Japanese white rabbits, male and female in half, were randomly divided into two groups, each group 24. A Group: common bile duct ligation group (BDL); B Group: BDL PNS group. the establishment of experimental animal models. in all experimental animals were kept under the same c