Paratyphoid specific transfer factor preparation and verification.doc

 PAGE \* MERGEFORMAT 9 Paratyphoid specific transfer factor preparation and verification Of: Jian-Wei Zhou, Luo Junmin, Sun Wanbang, Rui Ma, Tian Dan [Abstract] Objective To prepare specific transfer factor paratyphi (PA STF), for clinical assistance to provide emergency treatment to prevent or paratyphoid fever of the new biological agents. Methods immune prepared PA STF, by ultraviolet spectrophotometer PA STF multi-wavelength scanning, the black moss Lowry phenol method and improved PA STF were detected in the ribose and peptide content, and sterility test, Pyrogen test and safety tests, in order to immune protection of the immunological test activity. Results PA STF physical and chemical properties consistent with lt;”China Biological Productsgt;” (2000 version) of the required standard; immune protection experiments, mice fed PA STF survival rates were significantly higher than those fed small nTF and NS the survival rate of mice (P lt;0.05). Conclusion prepared PA STF, can be transferred for the preparation of Salmonella paratyphi A specific immune response in mice enhanced anti-Salmonella paratyphi immunity. [Keywords:] paratyphoid; specific transfer factor; preparation; test ABSTRACT: Objective To prepare a new kind of biological agent initially, which will be used for emergent prevention or adjuvant therapy for paratyphia.Methods Paratyphia specific factor (PA STF) was prepared in vivo and scanned with multi wavelength using ultraviolet spectrophotometer. Then we determined the content of polypeptide and ribose with orcinol assay and modified Lowry assay respectively, followed by sterility test, pyrogen test and safety test. The immunological activity was assayed by immune protection test.Results The physico chemical properties of PA STF accorded with the criteria of Chinese Bioproduct Rules (2000 edition). In the immune protection test, the survival rate of mice was higher in the two experiment groups than in control group and NS group (P