PEP1SOD1 fusion protein was transfected into the capacity of rat myocardium and its activity.doc

 PAGE \* MERGEFORMAT 2 PEP1SOD1 fusion protein was transfected into the capacity of rat myocardium and its activity Author: Zhang Yan, Wang Ning, Jun-Ming Tang, GUO Ling-Yun, YANG Jian-ye, HUANG Yong-Zhang, Zheng Fei, Kong Xia [Abstract] Objective: To study the PEP  1  SOD1 fusion protein transduction into rat heart capacity, and the purpose of determination of protease activity of transduction. Methods: Rats were divided into SOD1 group and PEP  1  SOD1 group, each of the two major mouse tail vein injection of 500 μ g purified SOD1, and 500 μ g purified PEP  1  SOD1 fusion protein into rats, after injection 0.5,1,2,4,8,24 h time point to take the heart (n = 6, each group at each time point) by 40 g / L paraformaldehyde solution, fixed 6 h Across frozen sections by immunohistochemistry to detect PEP  1  SOD1 into the capacity of myocardial tissue of rats and distribution. Determination of myocardial tissue xanthine oxidase activity of SOD1. Results: PEP  1  SOD1 group 0.5 h after injection, the rat myocardial tissue appears uniformly bright red fluorescent signal, distributed in the cytoplasm and the nucleus, with a time-dependent characteristics, the strongest point of the fluorescence intensity 1 h time point, while the SOD1 group did not see the red fluorescent signal. 0.5 h after injection when the SOD1 activity began to increase PEP  1  SOD1 group (85.37 ± 1.67), SOD1 group to (52.23 ± 0.67), compared two groups, the difference was statistically significant (P