Photodynamic therapy on human ovarian cancer cell line Skov3 apoptosis.docVIP

 PAGE \* MERGEFORMAT 13 Photodynamic therapy on human ovarian cancer cell line Skov3 apoptosis [Abstract] Objective by photodynamic therapy through (PDT) treatment of ovarian cancer cell apoptosis situation Skov3 study of photodynamic therapy on the proliferation of human ovarian cancer cells. Methods of different concentrations of different energy laser photosensitizer (5-ALA)-treated ovarian cancer cell lines (Skov3) were irradiated, 48 h after the application of light and electron microscopy were observed, but withered by flow cytometry death. Results inverted microscope after photodynamic treatment Skov3 cell shrinkage, cell deformation, shrinkage, nuclear condensation, stained chromatin into dense patches, cells lose their connection to each other by transmission electron microscopy shows dense Under the aggregation of chromatin along the nuclear membrane, formation of apoptotic bodies. maximum flow cytometry analysis of apoptosis was 60.5%. Conclusion PDT by inducing apoptosis in vitro Skov3 cells in vitro. [Key Words] optical dynamics; apoptosis; ovarian cancer [Abstract] Objective The apoptosis of ovarian cancer cell line Skov3 under the photodynamic therapy were studied to explore the effect of photodynamic therapy in gynecological cancer. Methods Ovarian cancer cell line Skov3 treated with different doses of photosensitizer 5 - ALA were radiated by laser with difference doses, then morphological characteristics were studies by light microscopy and electronic microscopy, and cell apoptosis were studied with flow cytometry (FCM). Results The cytomorphosis and shrinkage, nucleus condensation and anachromasia, and dense chromatin lining along the cell membrane were observed under inverted microscope in cell line received photodynamic therapy. Through transmission electron microseope (TEM), we found that nucleus chromatin gathering along membrane of cell nuclear and apoptotic body formed. The maximum inhibition rate was 60.5%, examined by FCM. Co