Phrenic nerve after nerve transposition diaphragm motor evoked potentials with Pathologic Correlation.docVIP

 PAGE \* MERGEFORMAT 2 Phrenic nerve after nerve transposition diaphragm motor evoked potentials with Pathologic Correlation Authors: Zhou Hui Xu, Jia-Shun, Yuan, Yan Wang Jun, Zhang Yong [Abstract] [Objective] To investigate the phrenic nerve reconstruction nerve transposition high respiratory function in rats after spinal cord injury in the diaphragm of the pathological changes and diaphragmatic motor evoked potentials (motion evoked potential, MEP) characteristics. [Method] 60 healthy male SD rats. Were randomly divided into 1 to 6 months 6 time groups. Remove the neck incision, the bilateral nerve at the level of the subclavian issued, the lateral branches before the cut, shift the start sewing then the Department of phrenic nerve trunk. After the first 1 to 6 months, samples of each group to take neck incision, excision C3 lamina fully reveal the whole spinal cord and in C3, 4 levels of sharp cross-section. Confirmed that the anterior tibial muscle MEP disappeared completely after the ribs on both sides of the anterior axillary line edge to make an incision reveals diaphragm abdominal side of the premises. Concentric needle electrodes under direct vision to the 9th rib on the anterior axillary line perpendicular to the lower edge of the diaphragm into the chest wall ribs, to monitor their MEP changes. Then remove the diaphragm integrity, electronic weighing scale. And in the right anterior axillary line shun the direction of diaphragm muscle fibers were cut 2 mm wide strips OK HE staining. Analysis of diaphragm muscle fiber cross-sectional area changes. [Results] nerve transposition with time after the prolonged incubation of rat diaphragm MEP progressively shorter and the amplitude is gradually increasing. 6-month group MEP amplitude (6.35 ± 0.51) mV, an incubation period (3.41 ± 0.36) ms. Meanwhile, the diaphragm gradually plump, muscular weight gradually restored, 6 months, the normal control group (97.23 ± 4.07)%. Muscle fiber cross-sectional ar