Pioglitazone on FFA TC3 cells under the action of the intervention of GPR40 expression.doc

Pioglitazone on FFA TC3 cells under the action of the intervention of GPR40 expression.doc

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Pioglitazone on FFA TC3 cells under the action of the intervention of GPR40 expression

 PAGE \* MERGEFORMAT 14 Pioglitazone on FFA TC3 cells under the action of the intervention of GPR40 expression [Abstract] Objective To investigate free fatty acid (FFA) TC 3 islet cells under the action of G protein receptor 40 (GPR40mRNA expression changes, and pioglitazone (Piog intervention of this change. Methods to study TC 3 cells were divided into control group and the FFA group (0.25,0.5, and 1 mmol / L. semi-quantitative RT PCR to detect the expression of GPR40. with different concentrations of Piog (0,0.1,1,10 mol / L pre-incubated TC 3 cells 6 h, adding 1 mmol / L FFA were incubated for 24 h, semi-quantitative RT PCR to detect the expression of GPR40. Results (1) FFA after 12 h incubation, the expression of GPR40 in each group no significant difference. (2) FFA after 24 h incubation with control group, 0.25 mmol / L FFA group was no difference in the expression of GPR40, but 0.5 mmol / L and 1 mmol / L FFA group GPR40 downregulated (P lt;0.05,0.5 mmol / L and 1 mmol / L FFA group GPR40 expression difference was not statistically significant. (3) and 1 mmol / L FFA group, 0.1 mol / L Piog +1 mmol / L FFA group of GPR40 mRNA expression was not different, and 1 mol / L Piog +1 mmol / L FFA group and 10 mol / L +1 mmol / L FFA group of GPR40 expression was increased (P lt;0.01. Conclusion Long-term effect of high concentrations of FFA could be reduced TC 3 cells the expression of GPR40, and Piog help protect or reduce the FFA level of abnormal result TC 3 GPR40 expression in cell damage. [Keywords:] thiazole TZDs, PPAR , fatty acids, diabetes, type 2, receptors, G protein-coupled of GPR40 in TC 3 cells induced by free fatty acids (FFA) and the intervention effect of pioglitazone (Piog) on those expressions. Methods TC 3 cells studied in the experiment were subject to different treatments with a blank and three concentrations of FFA (0.25 , 0.5 and 1.0 mmol / L). Semi quantitative RT PCR analysis was used to determine the RNA expression level of

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