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Phenol - sulfuric acid method Scorzonera roots of peach leaf polysaccharide
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Phenol - sulfuric acid method Scorzonera roots of peach leaf polysaccharide
[Abstract] Objective: To extract the roots of peach leaf Scorzonera determination of polysaccharides and polysaccharide. Methods: The decoction of the root extraction of polysaccharide Scorzonera peach leaf, using phenol - sulfuric acid method polysaccharide. Results: The boiling extraction Scorzonera roots of peach leaf polysaccharides was 13.9%. glucose concentrations in the tested range (0.03 ~ 0.08mg mL-1) a good linear relationship, r = 0.999 2. The average recovery was 101.7%, RSD = 0.61 %. bungeanus Scorzonera roots of the average content of polysaccharides 96.64 mg g-1, RSD = 0.10%. Conclusion: phenol - sulfuric acid method peach leaf polysaccharide Scorzonera roots feasible method is stable, simple.
[Keywords:] peach leaf Scorzonera; polysaccharide; spectrophotometry
[ABSTRACT] Objective: To extract Polysaccharides from Scorzonera sinensis Lipsch, Et Krasch and determine the content of polysaccharides.Methods: To extract Polysaccharides with decoction method.The contents of the Polysaccharides in Scorzonera sinensis Lipsch, Et Krasch were determined by Phenol-sulfurie acid method.Results: The extraction yield of Polysaccharides was 13.9%, and the standard curve was excellent liner in the concentration range of 0.03 ~ 0.08 mg mL-1 with correlation coefficient of r = 0.999 2.The recovery rates was 101.7%, RSD = 0.61%. The content of polysacharides from Scorzonera sinensis Lipsch, Et Krasch was 96.64mg g-1, RSD = 0.10%. Conclusion: The method is proved to be stable, feasible and simple.
[KEY WORDS] Scorzonera sinensis, Polysaccharides, Spectrophotometry
Tao Ye Tao Ye is a composite plant Scorzonera Scorzonera (Scorzonera sinensis Lipsch, Et Krasch) dry whole plant. Widely distributed in Hebei, Inner Mongolia, Shandong and other places. The public has the lizard feeding pipe oil (mainly nicotine) after with peach leaf milk Scorzonera repor
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