Phenol - sulfuric acid Quantitative determination of polysaccharides in Campanulaceae.doc

 PAGE \* MERGEFORMAT 17 Phenol - sulfuric acid Quantitative determination of polysaccharides in Campanulaceae Author: Li Yan, Wei and Xu Xudong Feng-Hua Shi CHU Qing-Long [Abstract] Objective To establish the quantitative determination of polysaccharide Campanulaceae method for the evaluation of Campanulaceae herbs and the new cultivar selection of quality provide a way. Methods Campanulaceae water samples extracted by the multi-stage treatment to remove impurities obtained Campanulaceae refined polysaccharide, refined polysaccharide dissolution and phenol - sulfuric acid reagents color; with refined polysaccharide measured Campanulaceae polysaccharide and glucose conversion factor, using phenol - sulfuric acid colorimetric method Campanulaceae polysaccharide samples. Resulted in the creation of a phenol - sulfuric acid mass fraction determination method of polysaccharide Kikyo, the average recovery was 97.54%, RSD = 1.44%. Platycodon different sources in the mass fraction of polysaccharide was determined, and its scope of 8.54% ~ 20.28%. Conclusion This method is simple and feasible, Campanulaceae polysaccharide within 4 h for the test solution in the color stability, good reproducibility can be used as Campanulaceae determination of polysaccharides. [Keywords:] Kikyo polysaccharide; phenol - sulfuric acid method; quantitative determination of Abstract: ObjectiveAn phenol-H2SO4 colorimetry method was developed for the determination of polysaccharides in Radix platycodonis samples from different origins, in order to provide a scientific basis for a quality evaluation of breeding good Radix platycodonis.MethodsExtracted with water, removed the interference components during the pretreatment process, and a corrected factor was used in order to minimize the error between glucose and polysaccharides of determination, the contents of the polysaccharides in Radix platycodonis samples were determined by phenol-H2SO4 colorimetry method.ResultsAn phenol-H2SO4 colori