Plasmodium gene transfection research.doc

 PAGE \* MERGEFORMAT 21 Plasmodium gene transfection research Keywords: Plasmodium falciparum; Plasmodium falciparum Pf; gene transfer; pathogenic organism With the Plasmodium falciparum (Plasmodium falciparum, Pf) 2 number [1], 9 [2] and 3 [3] chromosome sequences have clear, on the genome of Pf significant step forward in the next two ~ 3 years, it will be possible in all of its completed genome sequence analysis. There is no doubt that the parasite is imminent or has entered a research post-genomic era [4]. Pairs of parasite genes and gene products of various functions of research is to discover the role of effective vaccines and antimalarial drugs necessary prerequisite for target site, but the function of many genes, especially of important parasite virulence gene’s function has not yet been clear, thus affecting the malaria control work. Previous study of gene function limited to a comparison with the known functional genes and indirectly, to assume that in recent years the development of malaria parasite gene transfer technology [5] can be observed by changing the gene phenotype changes in vivo study of gene function, so that malaria parasite genes significantly changed the status quo. Not only that, the technology also enabled the parasite gene expression regulation, drug resistance studies, cell biology and vaccine studies have appeared in a new technical changes. In the four kinds of parasites in an infected person, Pf pathogenicity of the most serious attention devoted to its study has always been, this article on the parasite (mainly Pf) gene transfer studies are reviewed. A type of parasite gene transfer 1.1 The transient transfection Since the 70 late Hinnen et al [6] Since the establishment of yeast transfection, eukaryotic cells of transgenic technology in gene regulation and gene function studies play an extremely important role. 1993 Goonewardence, etc. [7] first carried out the parasite gene transfer pioneering work, for the parasite gen