Platelet activation and brachial-ankle Pulse Wave Velocity.doc

 PAGE \* MERGEFORMAT 6 Platelet activation and brachial-ankle Pulse Wave Velocity OF: Yes, Yanping Wang Lingling Yiwen Wei Lei dreamed Gongai Bin Wei Weirong [Keywords:] diabetes mellitus, platelet activation, CD41, CD62P, CD63, brachial-ankle pulse wave velocity Atherosclerosis is one of the main complications of diabetes, recent studies have reported increased platelet activation in the formation of atherosclerosis has an important role (1,2), platelet surface CD41 by flow cytometry , CD62P, CD63 expression of the amount, from the molecular level reflects the degree of platelet activation, is widely recognized as the assessment of a sensitive platelet activation marker (3), brachial-ankle pulse wave velocity (baPWV) in recent years to measure arterial Another new indicator sclerosis (4,5), but its relationship with platelet activation remains unclear. In this study, flow cytometry of platelet activation in patients with type 2 diabetes markers CD41, CD62P, CD63, and its association with baPWV relationship. 1 Materials and Methods 1.1 General Information Our hospital 60 patients with type 2 diabetes, disease duration (12.7 + -3.5), the fasting plasma glucose (7.6 + -2.8) mmol / L, HbA1c (7.4 + -3.3)%, which with large vascular disease (hypertension, coronary heart disease , cerebral arteriosclerosis) 30 patients, 18 males and 12 females, age (68.1 + -4.8) years, no major vascular disease group of 30 patients, 20 males and 10 females, age (67.5 + -3.9 years) normal control group of 30 patients, 19 males and 11 females, age (67.5 + -5.3 years) healthy subjects for the same period the diagnosis of Type 2 diabetes, the American Diabetes Association (ADA) 2005 diagnosed in standards. 1.2 Methods Fasting venous blood in all subjects 4 ml, produced by Beckman Coulter of Coulter Epics XL flow cytometry-based blood CD41, CD62P, CD63, Coulter antibody reagents are provided by the United States to collect 10 000 a platelet to the percentage of platel