PNPTK fusion suicide gene on hepatocellular carcinoma cell killing in rats.docVIP

 PAGE \* MERGEFORMAT 15 PNPTK fusion suicide gene on hepatocellular carcinoma cell killing in rats Of: Zhoujun Li, Cai Xiaokun Yang Yi Prohibition De-Quan Yao Zhenjiang [Abstract] Objective To analyze the PNP TK fusion suicide gene on HepG2 liver cancer cell killing and its effect on liver cancer cell killing mechanisms. Methods Preparation of recombinant PCR mutagenesis PNP TK fusion gene was inserted into the eukaryotic expression vector pcDNA3 .0 to construct the fusion gene expression vector pcDNA3.0/PNP TK, by restriction analysis, PCR and DNA sequencing of recombinant, G418 se