Podophyllotoxin solid lipid nanoparticles percutaneous penetration and skin of diluting.doc

 PAGE \* MERGEFORMAT 9 Podophyllotoxin solid lipid nanoparticles percutaneous penetration and skin of diluting [Abstract] Objective podophyllotoxin solid lipid nanoparticles of the percutaneous penetration and skin stay in storage. Methods Franz diffusion cell determination by solid lipid nanoparticles containing podophyllotoxin percutaneous penetration rate of diffusion between the pool and pool supplies for large mouse or rabbit abdominal skin diffusion cell area of 2.92 cm2. after the end of the in vitro penetration test, remove the skin, cut and homogenized with normal saline with 50% isopropanol extract, extract treated with the HPLC determination of drug concentrations . Results obtained in the podophyllotoxin solid lipid nanoparticles encapsulation averaged 95.28%, RSD was 2.53% (n = 5), diameter of between 50 ~ 200 nm. Solid lipid nanoparticles as a carrier when podophyllotoxin in vitro by rat and rabbit abdominal skin, the average infiltration rate and cumulative amount of more commercial tincture through significantly lower than the storage capacity of both drugs significantly improved commercial tinctures. Conclusion podophyllotoxin solid lipid nanoparticles with strong targeting the skin. [Keywords:] podophyllotoxin; solid lipid nanoparticles; percutaneous penetration; skin Storing Abstract: Objective To study in vitro transdermal penetration and retention of podophyllotoxin solid lipid nanoparticles (SLN). Methods The in vitro transdermal penetration of podophyllotoxin from SLN was determined by Franz diffusion cells. Between donor compartment and receptor compartment was abdominal skin of rats or rabbits , the avaiable diffusion area of cells was 2.92 cm2. The sample was analyzed by HPLC after treatment. After in vitro transdermal penetration experiment completed, the skin were taken down from cells, cut and homogenated, then extracted with 50% isopropanol normal saline. The extracting solution was analyzed by HPLC. Results The encapsulat