Polyethylene glycol-modified cholesterol esterase in high-density lipoprotein cholesterol determination of the role of homogeneous.docVIP

 PAGE \* MERGEFORMAT 3 Polyethylene glycol-modified cholesterol esterase in high-density lipoprotein cholesterol determination of the role of homogeneous [Abstract] Objective: To carry out modification of cholesterol esterase to evaluate the reaction selectivity-modifying enzymes. Methods: The agent on a variety of polyethylene glycol-modified cholesterol esterase in order to obtain a series of modifying enzymes, and then study the modifying enzymes in high-density lipoprotein cholesterol, the role of the clinical examination. Results: The arm polyethylene glycol-modified enzymes modifier modifier than with the other modified enzyme has better thermal stability and higher activity, and showed a higher high-density lipoprotein cholesterol selectivity. Conclusion: The surface-active agents on the high-density lipoprotein selective dissociation plays an important role, which will help modifying enzymes play its high-density lipoprotein cholesterol response to selective. [Keywords:] polyethylene glycol-modified enzymes of high-density lipoprotein cholesterol esterase agents selective dissociation Large number of epidemiological and clinical studies confirm that atherosclerosis (AS), coronary heart disease (CHD) incidence and serum high-density lipoprotein cholesterol (HDL  C) level was negatively correlated with the low-density lipoprotein cholesterol ( LDL  C) levels were positively correlated. Rapid and accurate determination of serum HDL  C and LDL  C levels on the clinical diagnosis is of great significance [1  3]. High-density lipoprotein cholesterol homogenous assay (HDL  C homogeneous methods) commonly known as the direct method (direct method), due to the sample without pretreatment, can be automated analyzer is suitable for large-scale epidemiological investigation, and the heavy workload of the laboratory use, with specimens with less, fast, simple and high precision advantages of [4]. Sugiuchi, etc. [5] developed a polyethylene glycol-modi