Polygonum multiflorum and its adulterants reaction sequence-related amplified polymorphism Optimization.doc

 PAGE \* MERGEFORMAT 7 Polygonum multiflorum and its adulterants reaction sequence-related amplified polymorphism Optimization [Abstract] Objective To establish a fleece-flower root and its adulterants sequence related amplified polymorphism (SRAP) molecular identification technology system. Orthogonal Methods impact of SRAP-PCR of Mg2, dNTPs, primer concentration and the amount of Taq polymerase. Results 20 μ l reaction system optimized combination of: Mg2 2.5 mmol / L, dNTPs 0.4 mmol / L, primer 0.35μ mol / L, Taq enzyme 1U. Conclusion Mg2, dNTPs, primer concentration and the amount of Taq polymerase on the SRAP-PCR results of different species have different effects, using SRAP molecular identification of four factors before this optimization. [Keywords:] Polygonum multiflorum Adulterant related amplified polymorphism sequence optimization Abstract: ObjectiveIn order to characterize Polygonum multiflorum Thunb. And its adulterants, the SRAP molecular marker system was established.MethodsOrthogonal design was used to optimize the concentrations of Mg2, dNTPs, primers and Taq DNA olymerase. ResultsThe optimum system of 20 μ l was as follows : Mg2 2.5 mmol / L, dNTPs 0.4 mmol / L, primer 0.35 μ mol / L, Taq DNA polymerase 1 U. ConclusionThe concentrations of Mg2, dNTPs, primers, Taq DNA olymerase have different effect in the result of SRAP-PCR in different species . It is necessary to optimize these four factors before using SRAP to identity different species. Keywords:: Polygonum multiflorum Thunb.; Adulterants; SRAP; Optimize Polygonum multiflorum Polygonum multilorum Thunb. Is a traditional Chinese precious Chinese medicine, Guangdong Ten ‘genuine medicine’ one. In recent years, its pharmacological efficacy research and development and application of more and more widely. According to the research reported that their main chemical constituents of medicinal anthraquinones, stilbene class, stilbene, polysaccharides, etc. [1 ~ 4]. Polygonum multiflorum e